Amino Acids, Peptides and Proteins (SPR Amino Acids, by J. H. Jones PDF

By J. H. Jones

ISBN-10: 1847557252

ISBN-13: 9781847557254

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Extra info for Amino Acids, Peptides and Proteins (SPR Amino Acids, Peptides, and Proteins (RSC)) (Vol 1)

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Spande and B. Witkop, Methods in Enzymology, 1967, 11, 498. l8 17a Structural Investigation of Peptides and Proteins 35 A study of the reaction of NBS with several proteins23 indicated that partial reaction of the tryptophan was occurring owing to the fact that some tryptophan residues are buried and some exposed in the native protein. A recent report 24 where the oxidation of lysozyme was evaluated by chemical, as well as spectral, analysis has suggested that it might not be quite as simple as that.

Gross, and J . Rudinger, Coll. Czech. Chem. , 1968, 33. 1307. 'Cherbuliez, 0. Espejo, B. Willhalm, and J. Rabinowitz, Helu. Chim. Actn, 1968, 51, 241. J. Leclerc and L. Benoiton, Cnnad. J. , 1968, 46, 1047. o-acids,Peptides, and Proteins a,y-diaminobutyric acid and a$-dianiinopropionic acid, however. 155 Tos I N NH II . e. 156 Studies l5’, 159 of the reaction of the ninhydrin analogue (45) with a-amino-acids have provided, by analogy, further corroboration of McCaldin’s mechanism lS9for the ninhydrin reaction.

1968, 107, 728. W. R. Gray, Methods in Enzymology, 1967, 11, 139. C. Gros and B. Labouesse, European J . , 1969, 7 , 463. D. Morse and B. L. Horecker, Analyt. , 1966, 14, 429. C. Gros, Bull. chim. France, 1967, 3952. 40 Amino-acids, Peptides, and Proteins DNS-amino-acids on thin-layer silica gel have been described, but Gray 67 warns that in his experiments with that technique there was a rapid destruction of dansyl amino-acids adsorbed on to silica. 61 Probably the best, and most sensitive, separation is currently achieved on polyamide layer sheets:62 10-lo mole of DNS-amino-acid can be detected with ease.

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Amino Acids, Peptides and Proteins (SPR Amino Acids, Peptides, and Proteins (RSC)) (Vol 1) by J. H. Jones

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